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| 14.06.2000 | Fortuna Düsseldorf | - | Hamburgo | 4:6 pn. | ||
| Blumenthaler SV | - | Hansa Rostock | 0:10 | |||
| Borussia Dortmund | - | Fortuna Köln | 2:1 | |||
| Bor. Mönchengladbach | - | Energie Cottbus | 4:1 | |||
| 1. FC Magdeburg | - | VfL Osnabrück | 5:2 | |||
| VfB Oldenburg | - | Wolfsburgo | 4:1 | |||
| Holstein Kiel | - | Reinickendorfer Füchse | 0:2 | |||
| Colonia | - | Hertha | 3:1 | |||
| 1. FC Saarbrücken | - | Mainz | 2:4 | |||
| Augsburgo | - | FK Pirmasens | 2:0 | |||
| SV Steiningen | - | Darmstadt | 1:7 | |||
| DJK Donaueschingen | - | TSV 1860 München | 0:6 | |||
| Rot-Weiß Hasborn | - | SSV Ulm 1846 | 10:11 pn. | |||
| FV Dresden-Nord | - | Karlsruher SC | 1:3 | |||
| 1. FC Nürnberg | - | Eintracht Frankfurt | 2:6 | |||
| Dynamo Dresden | - | Rot-Weiß Erfurt | 0:1 |
