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| 06.05.2022 | 17:00 | AC Sparta Praha B | - | SFC Opava | 1:2 (0:1) | |
| 17:30 | FC Sellier & Bellot Vlašim | - | FK Fotbal Třinec | 4:1 (1:0) | ||
| 07.05.2022 | 10:15 | MFK Vyškov | - | Vysočina Jihlava | 2:1 (1:1) | |
| 17:00 | 1. FK Příbram | - | SK Líšeň | 2:1 (1:1) | ||
| 17:00 | FK Ústí nad Labem | - | MFK Chrudim | 0:3 (0:0) | ||
| 08.05.2022 | 10:15 | Viktoria Žižkov | - | 1. SK Prostějov | 1:1 (0:1) | |
| 17:00 | Zbrojovka Brno | - | FK Dukla Praha | 2:1 (0:0) | ||
| 17:00 | FK Varnsdorf | - | FK MAS Táborsko | 2:0 (0:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Zbrojovka Brno | 28 | 22 | 2 | 4 | 58:25 | 33 | 68 |
| 2 |
|
FC Sellier & Bellot Vlašim | 28 | 15 | 4 | 9 | 57:37 | 20 | 49 |
| 3 |
|
SFC Opava | 28 | 13 | 9 | 6 | 44:30 | 14 | 48 |
| 4 |
|
SK Líšeň | 28 | 13 | 8 | 7 | 43:30 | 13 | 47 |
| 5 |
|
AC Sparta Praha B | 28 | 13 | 6 | 9 | 48:34 | 14 | 45 |
| 6 |
|
FK Varnsdorf | 28 | 12 | 5 | 11 | 43:35 | 8 | 41 |
| 7 |
|
Vysočina Jihlava | 28 | 10 | 8 | 10 | 27:33 | -6 | 38 |
| 8 |
|
FK Dukla Praha | 28 | 10 | 7 | 11 | 38:39 | -1 | 37 |
| 9 |
|
FK MAS Táborsko | 28 | 9 | 9 | 10 | 31:33 | -2 | 36 |
| 10 |
|
1. FK Příbram | 28 | 9 | 8 | 11 | 35:45 | -10 | 35 |
| 11 |
|
MFK Vyškov | 28 | 9 | 7 | 12 | 42:42 | 0 | 34 |
| 12 |
|
MFK Chrudim | 28 | 8 | 10 | 10 | 27:32 | -5 | 34 |
| 13 |
|
1. SK Prostějov | 28 | 10 | 4 | 14 | 28:44 | -16 | 34 |
| 14 |
|
FK Fotbal Třinec | 28 | 8 | 6 | 14 | 35:51 | -16 | 30 |
| 15 |
|
FK Ústí nad Labem | 28 | 5 | 10 | 13 | 23:45 | -22 | 25 |
| 16 |
|
Viktoria Žižkov | 28 | 2 | 9 | 17 | 26:50 | -24 | 15 |
| Ascenso | |
| Playoff | |
| Descenso |
