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| 22.04.2023 | 10:15 | MFK Chrudim | - | FK Třinec | 1:1 (1:1) | |
| 10:15 | MFK Vyškov | - | Slavia Praha B | 2:2 (1:1) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | FK Varnsdorf | 2:2 (2:1) | ||
| 23.04.2023 | 10:30 | AC Sparta Praha B | - | 1. SK Prostějov | 2:0 (0:0) | |
| 17:00 | Vysočina Jihlava | - | FK Dukla Praha | 0:1 (0:1) | ||
| 17:00 | FK MAS Táborsko | - | SFC Opava | 1:0 (0:0) | ||
| 17:00 | MFK Karviná | - | SK Líšeň | 2:2 (1:0) | ||
| 24.04.2023 | 16:00 | Sigma Olomouc B | - | FK Viagem Příbram | 4:1 (3:0) |
| » Clasificación | En casa | Fuera de casa | 1.ª vuelta | 2.ª vuelta |
| # | Equipo | P | V | E | D | Goles | DG | Pun. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
MFK Karviná | 24 | 13 | 4 | 7 | 43:33 | 10 | 43 |
| 2 |
|
SK Líšeň | 24 | 12 | 6 | 6 | 34:19 | 15 | 42 |
| 3 |
|
FK Viagem Příbram | 24 | 11 | 7 | 6 | 39:24 | 15 | 40 |
| 4 |
|
FK Varnsdorf | 24 | 11 | 6 | 7 | 43:31 | 12 | 39 |
| 5 |
|
FK Dukla Praha | 24 | 12 | 3 | 9 | 41:36 | 5 | 39 |
| 6 |
|
MFK Vyškov | 24 | 10 | 8 | 6 | 35:25 | 10 | 38 |
| 7 |
|
FC Sellier & Bellot Vlašim | 24 | 9 | 5 | 10 | 45:36 | 9 | 32 |
| 8 |
|
MFK Chrudim | 24 | 7 | 11 | 6 | 26:23 | 3 | 32 |
| 9 |
|
AC Sparta Praha B | 24 | 9 | 4 | 11 | 26:33 | -7 | 31 |
| 10 |
|
Sigma Olomouc B | 24 | 7 | 8 | 9 | 31:38 | -7 | 29 |
| 11 |
|
FK MAS Táborsko | 24 | 7 | 8 | 9 | 24:33 | -9 | 29 |
| 12 |
|
Vysočina Jihlava | 24 | 7 | 7 | 10 | 26:38 | -12 | 28 |
| 13 |
|
FK Třinec | 24 | 6 | 8 | 10 | 26:39 | -13 | 26 |
| 14 |
|
1. SK Prostějov | 24 | 6 | 8 | 10 | 31:45 | -14 | 26 |
| 15 |
|
Slavia Praha B | 24 | 6 | 7 | 11 | 32:45 | -13 | 25 |
| 16 |
|
SFC Opava | 24 | 5 | 8 | 11 | 20:24 | -4 | 23 |
| Ascenso | |
| Playoff | |
| Descenso |
