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| 01.09.2007 | 14:00 | Karlsruher SC | - | SC Regensburg | 0:1 | |
| 18:15 | SpVgg Rehweiler-Matzenbach | - | SV Dirmingen | 0:4 | ||
| 02.09.2007 | 11:00 | Energie Cottbus | - | Holstein Kiel | 0:9 | |
| 11:00 | TSV Jahn Calden | - | 1. FC Lok Leipzig | 1:3 | ||
| 11:00 | Erzgebirge Aue | - | SC Sand | 0:2 | ||
| 14:00 | Mellendorfer TV | - | FFC Oldesloe | 3:2 | ||
| 14:00 | FSV 02 Schwerin | - | Herforder SV | 2:9 | ||
| 14:00 | SuS Timmel | - | FC Gütersloh 2000 | 0:3 | ||
| 14:00 | SG Lütgendortmund | - | Victoria Gersten | 1:3 | ||
| 14:00 | FSC Mönchengladbach | - | TeBe Berlin | 0:2 | ||
| 14:00 | Geestemünder SC | - | 1. FC Union Berlin | 0:11 | ||
| 14:00 | Magdeburger FFC | - | SG Wattenscheid 09 | 1:6 | ||
| 14:00 | FC Bergedorf 85 | - | FFV Neubrandenburg | 3:8 | ||
| 14:00 | VfR Engen | - | ASV Hagsfeld | 0:4 | ||
| 14:00 | SV Eutingen | - | 1. FC Saarbrücken | 2:6 | ||
| 14:00 | SV Johannstadt 90 | - | FF USV Jena | 1:8 | ||
| 14:00 | 1. FFV Erfurt | - | TuS Niederkirchen | 2:6 | ||
| 14:00 | TuS Ahrbach | - | Wacker München | 1:2 | ||
| 14:00 | Fortuna Köln | - | TuS Köln rrh. | 1:3 | ||
| 15:00 | TSV Uengershausen | - | VfL Sindelfingen | 0:10 | ||
| 17:30 | VfR Limburg | - | FFC Brauweiler Pulheim | 1:0 |
