| 1. Jornada | 27.07.2009 | Bohemians 1905 | - | 1. FC Brno | 0:0 | ||||
| 2. Jornada | 02.08.2009 | 1. FC Brno | - | Slavia Praga | 2:0 | ||||
| 3. Jornada | 09.08.2009 | Slovan Liberec | - | 1. FC Brno | 0:1 | ||||
| 4. Jornada | 14.08.2009 | 1. FC Brno | - | 1. FC Slovácko | 1:0 |  |
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| 5. Jornada | 24.08.2009 | Baník Ostrava | - | 1. FC Brno | 1:1 | ||||
| 6. Jornada | 31.08.2009 | 1. FC Brno | - | FK Teplice | 2:3 | |
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| 7. Jornada | 13.09.2009 | SK Kladno | - | 1. FC Brno | 1:0 | ||||
| 8. Jornada | 21.09.2009 | 1. FC Brno | - | Sparta Praga | 1:1 | ||||
| 11. Jornada | 18.10.2009 | Sigma Olomouc | - | 1. FC Brno | 5:0 | ||||
| 12. Jornada | 24.10.2009 | 1. FC Brno | - | FK Baumit Jablonec | 0:1 | ||||
| 13. Jornada | 02.11.2009 | Viktoria Plzeň | - | 1. FC Brno | 1:1 | ||||
| 14. Jornada | 08.11.2009 | Bohemians Praha | - | 1. FC Brno | 2:1 | ||||
| 15. Jornada | 21.11.2009 | 1. FC Brno | - | Dynamo České Budějovice | 0:3 | ||||
| 16. Jornada | 29.11.2009 | Slavia Praga | - | 1. FC Brno | 3:1 |
República Checa