| 1. Jornada | 25.07.2009 | Slavia Praga | - | FK Mladá Boleslav | 1:1 | ||||
| 2. Jornada | 02.08.2009 | 1. FC Brno | - | Slavia Praga | 2:0 | ||||
| 3. Jornada | 09.08.2009 | Slavia Praga | - | Bohemians Praha | 3:1 | ||||
| 4. Jornada | 15.08.2009 | Dynamo České Budějovice | - | Slavia Praga | 0:1 | ||||
| 5. Jornada | 23.08.2009 | FK Teplice | - | Slavia Praga | 1:1 | ||||
| 6. Jornada | 31.08.2009 | Slavia Praga | - | 1. FC Slovácko | 3:0 | ||||
| 7. Jornada | 12.09.2009 | Slovan Liberec | - | Slavia Praga | 1:1 | ||||
| 8. Jornada | 21.09.2009 | Slavia Praga | - | Baník Ostrava | 3:1 | ||||
| 9. Jornada | 26.09.2009 | Bohemians 1905 | - | Slavia Praga | 1:1 |  |
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| 10. Jornada | 05.10.2009 | Slavia Praga | - | Sparta Praga | 0:1 | ||||
| 11. Jornada | 18.10.2009 | SK Kladno | - | Slavia Praga | 0:2 | ||||
| 12. Jornada | 26.10.2009 | Slavia Praga | - | Sigma Olomouc | 1:2 | ||||
| 13. Jornada | 31.10.2009 | 1. FK Příbram | - | Slavia Praga | 1:0 | ||||
| 14. Jornada | 09.11.2009 | Slavia Praga | - | Viktoria Plzeň | 0:0 | ||||
| 15. Jornada | 21.11.2009 | FK Baumit Jablonec | - | Slavia Praga | 1:1 | ||||
| 16. Jornada | 29.11.2009 | Slavia Praga | - | 1. FC Brno | 3:1 |
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