| 2. Jornada | 22.08.2016 | Vitesse (J) | - | Jong FC Twente | 1:3 | ||||
| 3. Jornada | 28.08.2016 | Jong FC Twente | - | TEC VV | 2:3 | ||||
| 6. Jornada | 17.09.2016 | GVVV | - | Jong FC Twente | 0:0 | ||||
| 12. Jornada | 05.11.2016 | Kozakken Boys | - | Jong FC Twente | 2:0 | ||||
| 13. Jornada | 12.11.2016 | Jong FC Twente | - | Excelsior Maassluis | 1:2 | ||||
| 14. Jornada | 20.11.2016 | Jong FC Twente | - | VV UNA | 1:3 |  |
|||
| 17. Jornada | 10.12.2016 | Jong FC Twente | - | Koninklijke HFC | 1:1 |
Austria